Cytochrome P450 2J2 Is Highly Expressed in Hematologic Malignant Diseases and Promotes Tumor Cell Growth

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Cytochrome P450 2J2 is highly expressed in hematologic malignant diseases and promotes tumor cell growth.

Cytochrome P450 2J2 (CYP2J2) epoxygenase converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EETs) that exert multiple biological effects in the cardiovascular system and in various human solid cancers. However, it is unknown whether this enzyme is expressed or plays any role in malignant hematological diseases. In this study, we found strong and highly selective CYP2J2 e...

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Carcinoma Cells and Is Up-regulated in Human Tumors Cytochrome P450 2J2 Promotes the Neoplastic Phenotype

Cytochrome P450 (CYP) arachidonic acid epoxygenase 2J2 converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids, which exert diverse biological activities in cardiovascular system and endothelial cells. However, it is unknown whether this enzyme highly expresses and plays any role in cancer. In this study, we found that very strong and selective CYP2J2 expression was detected i...

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Expression of cytochrome P450 arachidonic acid epoxygenase 2J2 in human tumor tissues and cell lines.

BACKGROUND AND OBJECTIVE Cytochrome P450 arachidonic acid epoxygenase 2J2 (CYP2J2) is a new metabolic pathway of arachidonic acid. However, its biological effects, especially pathophysiologic significance in human beings, remain to be further recognized. This study was to determine the expression of CYP2J2 in human tumor tissues and cell lines. METHODS The expression of CYP2J2 mRNA and protei...

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Expression and Characterization of Truncated Recombinant Human Cytochrome P450 2J2

The human cytochrome P450 2J2 catalyzes an epoxygenase reaction to oxidize various fatty acids including arachidonic acid. In this study, three recombinant enzyme constructs of P450 2J2 were heterologously expressed in Escherichia coli and their P450 proteins were successfully purified using a Ni(2+)-NTA affinity column. Deletion of 34 amino acid residues in N-terminus of P450 2J2 enzyme (2J2-D...

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ژورنال

عنوان ژورنال: Journal of Pharmacology and Experimental Therapeutics

سال: 2010

ISSN: 0022-3565,1521-0103

DOI: 10.1124/jpet.110.174805